There was a strong (hydrolysis) zone among all four bacterial isolates, indicating the existence of pectinase activities. [QxMD MEDLINE Link]. According to the investigations by Mohandas et al., [36] the highest pectinase activity, 2.43 U/ml, was recorded at alkaline pH 8 byBacillus sonorensis. Positive (+ve) S. Over the last 30 years, Serratia marcescens has become an important cause of nosocomial infection. This result was similar to that of [37, 52], which was done on the clarification of apple juice by pectinase. SIM medium should be read after an incubation of only 24 hours because a longer incubation time can cause a false negative. Through comparing the results of 18 Maktouf S, Neifar M, Drira SJ, Baklouti S, Fendri M, Chabouni SE. Sittidilokratna C, Suthirawut S, Chitradon L, Punsuvon V, Vaithanomsat P, Siriacha P. Screening of pectinase producing bacteria and their efficiency in biopulping of paper mulberry bark. WebWhen P. vulgaris is tested using the API 20E identification system [1] it produces positive results for sulfur reduction, urease production, tryptophan deaminase production, indole production, sometimes positive gelatinase activity, and saccharose fermentation, and negative results for the remainder of the tests on the testing strip. The use of cefepime for treating AmpC -lactamase-producing Enterobacteriaceae. Share cases and questions with Physicians on Medscape consult. Enterobacter cloacae exhibits fermenation of glucose and gas production but no sulfur reduction. The mixture of 1g avocado peel waste sample and 9ml of distilled water were serially diluted from 101 to 106 in test tubes [54]. Embaby AM, Masoud AA, Marey HS, Shaban NZ, Ghonaim TM. Similarly, the volume of juice variation from fruit to fruit that was treated with pectinase was investigated by using different fruits such as strawberry juice (7.0 10.0ml), grape juice (15.0 -21.5ml), apple juice (12.018.5ml), peach apple juice (12.010.5ml), chary apple juice (8.0 10.0ml) and orange juice (9.0 10.0ml) [29, 48]. to the surface of the tube. Acquisition of multidrug-resistant Serratia marcescens by critically ill patients who consumed tap water during receipt of oral medication. Arabinose Carrero P, Garrote JA, Pacheco S, et al. To figure out how much juice was made from each piece of fruit, Rai et al., [20] used the method. Those bacterial isolates with a higher clear area were subjected to submerged fermentation for pectinase production using the same medium as primary screening but without agar. endobj Steppberger K, Walter S, Claros MC, Spencker FB, Kiess W, Rodloff AC, Vogtmann C. Infection. Harris PN, Wei JY, Shen AW, Abdile AA, Paynter S, Huxley RR, et al. The tube was agitated for 30-60 seconds. endobj Serratia marcescens: historical perspective and clinical review. Pink hypopyon: a sign of Serratia marcescens endophthalmitis. Food Technol Biotechnol. change in color several minutes after the reagent had been added. 2022 Dec 5;11(12):1473. doi: 10.3390/pathogens11121473. The broth also includes dextrose. served as a selective media. Kazemi M, Khodaiyan F, Hosseini SS, Najari Z. In this analysis, the optimum fermentation period for pectinase development was found to be 72h, and the highest pectinase activities were found to be 6.860.32mol/ml/min of released glucose [Table 4] when pectin was used as a substrate. Antonette B Climaco, MD is a member of the following medical societies: American Academy of HIV Medicine, HIV Medicine Association, Infectious Diseases Society of America, Philippine Medical AssociationDisclosure: Nothing to disclose. Koshy M, De S. Effect of Bacillus tequilensis SALBT crude extract with pectinase activity on demucilation of coffee beans and juice clarification. HW _Q7$@HE= 5rX1 "QY|z}\x)4M]$w/?ly8@+r0T9POW%t_Vb {O{JJ'0=[ScB}i7a!}l'^# PubMed The inoculum was transferred into the sterile bacteremia in Canberra, Australia: a population-based study over 10 years. 2007 Nov. 39(9):2879-82. Premature termination of nursing secondary to Serratia marcescens breast pump contamination. medium was used. Serratia marcescens. Waste Manage. FOIA The purified pectinase was applied in the fruit juice-making process to test the clarity of the juice. Bergey's Manual of Determinative Bacteriology, based on their physical and biochemical properties, the ABIS-online software was used to detect Pectinolytic bacteria [27, 29]. Serratia infection is responsible for about 2% of nosocomial infections of the bloodstream, lower respiratory tract, urinary tract, surgical wounds, and skin and soft tissues in adult patients. Lemon, mango, and apple fruits were washed carefully and chopped into smaller sizes on the side with a sharp knife. Bergey's Manual of Determinative Bacteriology. [QxMD MEDLINE Link]. Chang CC, Chen WC, Ho TF, Wu HS, Wei YH. 2014;239(1):12735. 2013 Jul;51(7):2295-302. doi: 10.1128/JCM.03280-12. The results were compared to the given unknown results. These results indicate that S. marcescens have latent ability to infect and circulate between both plants and animals. Anal Chem. 2009;14:444554. the light pink background of the media. Pectinase activity increased gradually over 24h of incubation until optimum pectinase activity was achieved. Phytoparasitica. Cases of Serratia septic arthritis have been reported in patients receiving intra-articular injections, individuals with joint trauma, and patients with intravascular devices or who are undergoing intravascular procedures. It also speeds up tea fermentation and eliminates the foam-forming property of instant tea powder by destroying the pectin present in tea powder. 2015;15(59):1. https://doi.org/10.1042/bse0590001. As shown in Table 11, pectinase activities can change the phenolic compound profiles and the decomposition of another antioxidant by pectin hydrolysis [35, 52, 53]. 2022;23(3):1680. https://doi.org/10.3390/ijms23031680. Food Chemistry. Thomas E Herchline, MD Professor of Medicine, Wright State University, Boonshoft School of Medicine; Medical Consultant, Public Health, Dayton and Montgomery County (Ohio) Tuberculosis Clinic Catalysts. Google Scholar. Sodium chloride maintains osmotic equilibrium. Edited By: Sagar Aryal. The result of juice clarity treated with crude and purified pectinase was 1.99% and 18.82% more excellent than the previous report by Maktouf et al., [49], which was 77% of transmittance for pectinase using lemon fruit as the raw material for juice clarification. Figure 1. Clin Infect Dis. J Burn Care Res. Essays Biochem. The organism is not capable of fermenting arabinose which results in the formation of acidic end the intermediates of the fermentation pathway. 2001;17(1):7982. Enzymatic Reactions. John L Brusch, MD, FACP is a member of the following medical societies: American College of Physicians, Infectious Diseases Society of AmericaDisclosure: Nothing to disclose. All tests were performed in triplicates, and data were expressed as Meanstandard deviation. sodium salt as the sole source of carbon. The Folin-Ciocalteu technique was used to determine each fruit juice sample [23, 25]. Therefore, apple fruit showed consistent variation from control to crude and crude to purified pectinase. 344(20):1491-7. Faro J, Katz A, Berens P, Ross PJ. The medium is a nutrient broth to which 0.5-1.0% sucrose is added. Br A, Schmitt-Groh S, Held J, Lubig J, Hanslik G, Fahlbusch FB, Reutter H, Woelfle J, van der Donk A, Schleier M, Hepp T, Morhart P. Antibiotics (Basel). Pulmonary infection with serratia marcescens. This confirms a negative result, meaning, Serratia As with the phenol red fermentation broths, if an organism can ferment any of the three sugars present in the medium, the medium will turn yellow. Article Similarly, the results of each isolate's secondary screening method are more similar to the previous investigation of decomposing orange peels; this resulted in the sources of the samples mentioned by [22, 31]. }Q;4$G9luo='w|g jNs(&4B|ytMc7-.HUp0=(/p2uKh;Z@QZ|s/ t$( }z1LqRdu:qNk=R/(`J@YzC+ Hc}#WptQ8 ZeHw%3.gK,i/I9_`^?:w]^. The uses of these enzymes are depicted in Fig. Sci Asia. (Z6WG) isolated from the guts of termites (Isoptera). The amount of mango juice increased twofold from control to crude pectinase enzyme (8.010.0ml) after 1h of pectinase and water treatment and increased almost threefold from crude pectinase to purified pectinase (10.0 -13.5ml), and 66.67% of yield. [13]. 2009 Dec. 16(6):512-4. there was no gas production. ;&[wB2N4";UF. compound will turn red. Temperature is one of the essential parameters essential for the success of pectinase production. endobj Positive (+ve) Citrate. WebOptimization of pH for pigment production in Serratia marcescens The effects of different pH ranges from 5 - 9 were studied for the production of pigments. Kashyap DR, Vohra PK, Chopra S, Tewari R. Applications of pectinases in the commercial sector: a review. 11. Serratia marcescens is negative for acid production on lactose, but positive glucose and sucrose (with gas production) fermentation. Nitrate tests are positive since nitrate is generally used as the final electron acceptor rather than oxygen . Citrate (positive test) is used by Serratia marcescens to produce pyruvic acid. [QxMD MEDLINE Link]. 18 0 obj<> In the early part of the 20 th century, Serratia marcescens was considered a nonpathogenic organism and was used in medical experiments and as a biological warfare [QxMD MEDLINE Link]. Pigment (prodigiosin) biosynthesis by S. marcescens has been investigated fully since the emergence of the organism as a cause of infection. The ABTS and DPPH radical scavenging activities of each fruit juice sample were determined using a modified version of the method published by Hosseini et al., [53]. Provided by the Springer Nature SharedIt content-sharing initiative. RAPD-PCR has also been applied to a small number of isolates and seems to be a promising method, especially for rapid monitoring of an outbreak and tracing the source of initial infection. The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request. Staphylococcus saprophyticus | Osmosis Skin ulcers and disseminated abscesses are characteristic of Serratia marcescens infection in older patients with chronic granulomatous disease. A simultaneous outbreak of Serratia marcescens and Klebsiella pneumoniae in a neonatal intensive care unit. <>stream Selective and Differential Media - MacConkey, EMB Molecular characterization of microorganisms in municipal solid waste for production of industrial enzymes and enhanced biodegradation.
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